Structure-function analysis of human transforming growth factor-α by site-directed mutagenesis

Structure-function analysis of human transforming growth factor-alpha by site-directed mutagenesis.

Site-directed mutants of transforming growth factor-alpha (TGF-alpha) were expressed in an Escherichia coli outer membrane protein A (ompA) expression/secretion vector under the transcriptional control of the lambda PL promoter. TGF-alpha mutant proteins were isolated from cell pellets using alkaline extraction with 0.1 M-Tris (pH 10.5). The levels of protein expression of 23 TGF-alpha mutants ...

Site-Directed Mutagenesis in Human Granulocyte-colony Stimulating Factor, Cloning and Expression in Escherichia coli

Human granulocyte colony stimulating factor (hG-CSF) induces proliferation and differentiation of granulocyte progenitor cells. This glycoprotein is currently being used for treatment of neutropenia, in patients who have undergone bone marrow transplantation. So far, different researchers have tried to enhance hG-CSF biological activity and stability. In this study, Polymerase Chain Reaction (P...

site-directed mutagenesis in human granulocyte-colony stimulating factor, cloning and expression in escherichia coli

human granulocyte colony stimulating factor (hg-csf) induces proliferation and differentiation of granulocyte progenitor cells. this glycoprotein is currently being used for treatment of neutropenia, in patients who have undergone bone marrow transplantation. so far, different researchers have tried to enhance hg-csf biological activity and stability. in this study, polymerase chain reaction (p...

Structure-function studies on human retinol-binding protein using site-directed mutagenesis.

Retinol-binding protein (RBP) transports vitamin A in the plasma. It consists of eight anti-parallel beta-strands (A to H) that fold to form an orthogonal barrel. The loops connecting the strands A and B, C and D, and E and F form the entrance to the binding site in the barrel. The retinol molecule is found deep inside this barrel. Apart from its specific interaction with retinol, RBP is involv...

Biochemical Analysis of Halophilic Dehydrogenases Altered by Site-Directed Mutagenesis